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primary antibodies against v5 tag  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibodies against v5 tag
    Primary Antibodies Against V5 Tag, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+against+v5/HA-Tag+Rabbit+mAb/pm41241038-98-12-17
    Average 99 stars, based on 4944 article reviews
    primary antibodies against v5 tag - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Focal adhesion kinase promotes ribosome biogenesis to drive advanced thyroid cancer cell growth and survival
    Article Snippet: .. 15,000-40,000 cells were grown on 8-well chamber slides for 48 hours prior to fixation with 4% paraformaldehyde (PFA) (Invitrogen) for 15 minutes, permeabilization with 0.5% Triton-X (Sigma) for 10 minutes, blocking with 5% normal goat serum (NGS) (Invitrogen), and incubation with primary antibodies against V5 (Cell Signaling), HA (Cell Signaling), Total FAK (BD Biosciences), pY397 FAK (Invitrogen), and fibrillarin (Invitrogen) overnight at 4°C. .. Slides were then incubated for one hour with Alexa Fluorophores 488 Goat Anti-Rabbit (Invitrogen) at 1:500 and Alexa Fluor 555 Goat Anti-Mouse (Invitrogen) at 1:500 dilution followed by staining with DAPI (3 μg/mL) (Invitrogen) for 30 minutes.

    Next-Generation Sequencing:

    Article Title: Focal adhesion kinase promotes ribosome biogenesis to drive advanced thyroid cancer cell growth and survival
    Article Snippet: .. 15,000-40,000 cells were grown on 8-well chamber slides for 48 hours prior to fixation with 4% paraformaldehyde (PFA) (Invitrogen) for 15 minutes, permeabilization with 0.5% Triton-X (Sigma) for 10 minutes, blocking with 5% normal goat serum (NGS) (Invitrogen), and incubation with primary antibodies against V5 (Cell Signaling), HA (Cell Signaling), Total FAK (BD Biosciences), pY397 FAK (Invitrogen), and fibrillarin (Invitrogen) overnight at 4°C. .. Slides were then incubated for one hour with Alexa Fluorophores 488 Goat Anti-Rabbit (Invitrogen) at 1:500 and Alexa Fluor 555 Goat Anti-Mouse (Invitrogen) at 1:500 dilution followed by staining with DAPI (3 μg/mL) (Invitrogen) for 30 minutes.

    Incubation:

    Article Title: Focal adhesion kinase promotes ribosome biogenesis to drive advanced thyroid cancer cell growth and survival
    Article Snippet: .. 15,000-40,000 cells were grown on 8-well chamber slides for 48 hours prior to fixation with 4% paraformaldehyde (PFA) (Invitrogen) for 15 minutes, permeabilization with 0.5% Triton-X (Sigma) for 10 minutes, blocking with 5% normal goat serum (NGS) (Invitrogen), and incubation with primary antibodies against V5 (Cell Signaling), HA (Cell Signaling), Total FAK (BD Biosciences), pY397 FAK (Invitrogen), and fibrillarin (Invitrogen) overnight at 4°C. .. Slides were then incubated for one hour with Alexa Fluorophores 488 Goat Anti-Rabbit (Invitrogen) at 1:500 and Alexa Fluor 555 Goat Anti-Mouse (Invitrogen) at 1:500 dilution followed by staining with DAPI (3 μg/mL) (Invitrogen) for 30 minutes.



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    pY397 FAK localizes to the nucleolus and influences nucleolar FAK accumulation (A) BCPAP, 8505C, and KTC2 cells were stained <t>with</t> <t>antibodies</t> against pY397 FAK, FBL, and DAPI prior to confocal imaging at 100X. (B) BCPAP, 8505C cells, and KTC2 cells were probed with antibodies against total FAK and DAPI prior to imaging at 100X confocal. Results shown are representative images from 20–30 images taken in at least two independent experiments. Scale bar represents 20μM. (C) FAK-KO cells transduced with EV, N-terminal NLS FAK, and N-terminal NLS-Y397F were probed with antibodies against <t>V5,</t> Fibrillarin, and DAPI prior to imaging at 100X on Olympus Confocal. At least 50 cells were imaged in three independent experiments. Scale bars represent 5μm. (D) Percent of co-localization between V5 and FBL in NLS versus NLS-Y397F KO-FAK transduced cells using Integrated Density Analysis by Image J. Results displayed as mean +/- SEM. *, p<0.05.
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    Image Search Results


    pY397 FAK localizes to the nucleolus and influences nucleolar FAK accumulation (A) BCPAP, 8505C, and KTC2 cells were stained with antibodies against pY397 FAK, FBL, and DAPI prior to confocal imaging at 100X. (B) BCPAP, 8505C cells, and KTC2 cells were probed with antibodies against total FAK and DAPI prior to imaging at 100X confocal. Results shown are representative images from 20–30 images taken in at least two independent experiments. Scale bar represents 20μM. (C) FAK-KO cells transduced with EV, N-terminal NLS FAK, and N-terminal NLS-Y397F were probed with antibodies against V5, Fibrillarin, and DAPI prior to imaging at 100X on Olympus Confocal. At least 50 cells were imaged in three independent experiments. Scale bars represent 5μm. (D) Percent of co-localization between V5 and FBL in NLS versus NLS-Y397F KO-FAK transduced cells using Integrated Density Analysis by Image J. Results displayed as mean +/- SEM. *, p<0.05.

    Journal: Frontiers in Oncology

    Article Title: Focal adhesion kinase promotes ribosome biogenesis to drive advanced thyroid cancer cell growth and survival

    doi: 10.3389/fonc.2025.1252544

    Figure Lengend Snippet: pY397 FAK localizes to the nucleolus and influences nucleolar FAK accumulation (A) BCPAP, 8505C, and KTC2 cells were stained with antibodies against pY397 FAK, FBL, and DAPI prior to confocal imaging at 100X. (B) BCPAP, 8505C cells, and KTC2 cells were probed with antibodies against total FAK and DAPI prior to imaging at 100X confocal. Results shown are representative images from 20–30 images taken in at least two independent experiments. Scale bar represents 20μM. (C) FAK-KO cells transduced with EV, N-terminal NLS FAK, and N-terminal NLS-Y397F were probed with antibodies against V5, Fibrillarin, and DAPI prior to imaging at 100X on Olympus Confocal. At least 50 cells were imaged in three independent experiments. Scale bars represent 5μm. (D) Percent of co-localization between V5 and FBL in NLS versus NLS-Y397F KO-FAK transduced cells using Integrated Density Analysis by Image J. Results displayed as mean +/- SEM. *, p<0.05.

    Article Snippet: 15,000-40,000 cells were grown on 8-well chamber slides for 48 hours prior to fixation with 4% paraformaldehyde (PFA) (Invitrogen) for 15 minutes, permeabilization with 0.5% Triton-X (Sigma) for 10 minutes, blocking with 5% normal goat serum (NGS) (Invitrogen), and incubation with primary antibodies against V5 (Cell Signaling), HA (Cell Signaling), Total FAK (BD Biosciences), pY397 FAK (Invitrogen), and fibrillarin (Invitrogen) overnight at 4°C.

    Techniques: Staining, Imaging, Transduction